Staff Research Associate - 140910

The Staff Research Associate will carry out research under the direction of the Principal Investigator (PI) on lung development, regeneration, aging and disease in the mouse.

Incumbent will manage lab flow cytometry and cell sorting capabilities; run fluorescence-activated cell sort (FACS) and ex vivo 3D co-culture assays in the evaluation and fibroblast contribution to epithelial progenitor growth and differentiation. Incumbent will also operate and perform standard studies on a FACS for various PIs (Principal Investigators) within the Division of Biological Sciences.

Additionally, incumbent will generate data, analyze results, and present results at lab meetings. This position is also responsible for organizing and maintaining the lab, monitoring lab expenditures, and serves as lab biohazard and safety coordinator. Incumbent will train and manage undergraduate research assistants and assist others in their experiments. Perform other duties as assigned.

• Theoretical and practical knowledge of biochemistry, molecular biology, immunology and genetics, including relevant laboratory techniques, acquired through a BS in Biology or related field OR an equivalent combination of education and laboratory experience.

• Experience working with infectious agents of BL2/BL2+ using in vivo models.

• Proven ability to independently participate in the execution of research projects or portions of such projects, independently planning, organizing and performing experiments and selecting data for analysis.

• Experience in sterile technique for the propagation of prokaryotic and mammalian cells.

• Extensive knowledge of specialized scientific terminology relating to topics of molecular biology, cell biology and animal physiology.

• Experience working with mice as research model system, including experience genotyping, maintaining mouse lines, and performing surgery and dissection.

• Proven ability to utilize scientific literature and apply to a research project. Experience conducting scientific literature searches, including online databases and other resources.

• Ability to develop, follow and troubleshoot established lab protocols and lab procedures with direction from PI.

• Excellent organizational skills including the ability to keep clear and accurate records, organize a varied workload, follow written and oral protocols and give oral presentations on the progress of research in lab meetings. Proven ability to prioritize and schedule experiments and duties to maximize efficiency.

• Excellent interpersonal communication skills to interact well with others to facilitate communication about lab requirements including the ability to discuss and understand day-to-day laboratory affairs, research plans, results and ideas with PI and other lab personnel in a constructive, professional and pleasant manner.

• Familiarity with Apple and PC computers and associated word processing and spreadsheet software with a willingness and ability to update and increase skills and knowledge as needed.

• Demonstrated ability to hire and manage undergraduate student employees. Skill to train others in various lab techniques and procedures (safety precautions, preparation of media, dishwashing, equipment usage. laboratory maintenance, etc.).

• Knowledge/understanding and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Program. Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items. Willingness and ability to keep safety training current and consistent with research involvement.

• Experience and knowledge of proper handling, storage and disposal of chemicals, biochemicals, hazardous chemicals, and biohazardous materials including radioactive materials.

• Willingness and demonstrated ability to embrace and consistently uphold the principles of community for a collaborative work environment.

• Knowledge of laws and regulations that govern the housing and management of laboratory animals as applied to the University of California. Sensitivity to the needs of caged laboratory animals in compliance with AAALAC standards.

• Skill and knowledge to proactively maintain an organized lab and stocks of essential supplies and media, purchase new equipment, and ensure lab equipment is operating effectively.

• Ability to maintain confidentiality in matters related to work.

• Skill and knowledge to proactively maintain an organized lab and stocks of essential supplies and media, purchase new equipment, and ensure lab equipment is operating effectively.

• Must be willing to work with mice and be comfortable with the use of mice as experimental animals.

• Must be willing and able to work safely with BL2 level viral and known carcinogens used for gene activation.

• Must be willing to use various lung injury models.

• Flexible work schedule required. Willingness and ability to work occasional evenings and/weekends as required by the needs to the research project(s).

• Must be able to work on feet all day and willing and able to safely lift mouse cages.